Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor

YAMILE JALIT; FABIANA A. GUTIERREZ; GALINA DUBACHEVA; CEDRIC GOYER; LILIANE COCHE-GUERENTE; ERIC DEFRANCQ; PIERRE LABBE; GUSTAVO A. RIVAS; MARCELA C. RODRIGUEZ

ELSEVIER ADVANCED TECHNOLOGY

Lugar: Amsterdam; Año: 2013 vol. 41 p. 424 - 424

p style="TEXT-ALIGN: justify; LINE-HEIGHT: 150%; TEXT-INDENT: 35.4pt; MARGIN: 0cm 0cm 0pt; TEXT-AUTOSPACE: ; mso-layout-grid-align: none" class="MsoNormal">This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance